Strategies for the Assessment of Protein Aggregates in Pharmaceutical Biotech Product Development

Publication Type:

Journal Article

Source:

Pharmaceutical Research, Volume 28, Number 4, pp. 920-933 (2011)

ISBN:

0724-8741

DOI Name (links to online publication)

10.1007/s11095-010-0297-1

Keywords:

aggregation; aggregates; analytical characterization; biotech products; commentary; drug development; particles; proteins; recommendation; size-exclusion chromatography; field-flow fractionation; velocity analytical ultracentrifugation; heterogeneous nucl

Abstract:

Within the European Immunogenicity Platform (EIP) (http://www.e-i-p.eu), the Protein Characterization Subcommittee (EIP-PCS) has been established to discuss and exchange experience of protein characterization in relation to unwanted immunogenicity. In this commentary, we, as representatives of EIP-PCS, review the current state of methods for analysis of protein aggregates. Moreover, we elaborate on why these methods should be used during product development and make recommendations to the biotech community with regard to strategies for their application during the development of protein therapeutics.

21/04/2011